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Histone H3K14me1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Wide Range Predicted

Applications

IF, WB, Dot Blot

Cat No : 61509,61510 61509

Synonyms

Histone H3K14me1, Histone, H3, Lysine 14, K14, monomethyl, methylation, histone methylation, antibody, antibodies, polyclonal, pab, wb, western blotting, sample

验证数据展示

  • Histone H3K14me1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with H3K14me1 antibody at a 1:500 dilution.

    Histone H3K14me1 antibody (pAb) tested by Western blot. Nuclear extract of HeLa cells (20 ug) probed with H3K14me1 antibody at a 1:500 dilution.

  • Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H3K14me1 antibody (pAb) for monomethyl-lysine 14 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1:30,000. The amount of peptide (in picomoles) spotted is indicated next to each row.Top panel - Lane 1: unmodified Lys14. Lane 2: monomethyl Lys14. Lane 3: dimethyl Lys14. Lane 4: trimethyl Lys14. Middle panel - Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: unmodified Lys79. Lane 10: monomethyl Lys79. Lane 11: dimethyl Lys79. Lane 12: trimethyl Lys79. Bottom panel - Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.

    Specificity Data: Dot blot analysis was used to confirm the specificity of Histone H3K14me1 antibody (pAb) for monomethyl-lysine 14 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1:30,000. The amount of peptide (in picomoles) spotted is indicated next to each row.Top panel - Lane 1: unmodified Lys14. Lane 2: monomethyl Lys14. Lane 3: dimethyl Lys14. Lane 4: trimethyl Lys14. Middle panel - Lane 1: unmodified Lys4. Lane 2: monomethyl Lys4. Lane 3: dimethyl Lys4. Lane 4: trimethyl Lys4. Lane 5: unmodified Lys9. Lane 6: monomethyl Lys9. Lane 7: dimethyl Lys9. Lane 8: trimethyl Lys9. Lane 9: unmodified Lys79. Lane 10: monomethyl Lys79. Lane 11: dimethyl Lys79. Lane 12: trimethyl Lys79. Bottom panel - Lane 1: Unmodified Lys23. Lane 2: Monomethyl Lys23. Lane 3: Dimethyl Lys23. Lane 4: Trimethyl Lys23. Lane 5: unmodified Lys27. Lane 6: monomethyl Lys27. Lane 7: dimethyl Lys27. Lane 8: trimethyl Lys27. Lane 9: unmodified Lys36. Lane 10: monomethyl Lys36. Lane 11: dimethyl Lys36. Lane 12: trimethyl Lys36.

  • Detection of H3K4me1 by immunofluorescence U2OS cells were stained with H3K4me1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H3K4me1 antibody staining. Right panel: merge.

    Detection of H3K4me1 by immunofluorescence U2OS cells were stained with H3K4me1 antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H3K4me1 antibody staining. Right panel: merge.

产品信息

Tested Applications IF, WB, Dot Blot

Applications Validated by Active Motif: WB*: 1:500 - 1:2,500 dilution IF: 1:500 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.
Tested Reactivity Human, Wide Range Predicted
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationMethylated
Immunogen This antibody was raised against a peptide including monomethyl-lysine 14 of human histone H3.
Full Name Histone H3K14me1 antibody (pAb)
Synonyms Histone H3K14me1, Histone, H3, Lysine 14, K14, monomethyl, methylation, histone methylation, antibody, antibodies, polyclonal, pab, wb, western blotting, sample
Molecular weight 17 kDa
GenBank accession numberNP_003522
RRIDAB_2793664
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.