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Recombinant Human B7-1/CD80 protein (hFc Tag)

ED50

1-4 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

941

Accession

P33681-1

Cat No : Eg0967

Print datasheet

Synonyms

B7, B7-1, BB1, CD28LG, CD28LG1, CD80, LAB7

验证数据展示

  • Purity of Recombinant Human B7-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human B7-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CTLA-4 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human B7-1 (hFc tag) with a linear range of 1-4 ng/mL.

    Immobilized Human CTLA-4 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human B7-1 (hFc tag) with a linear range of 1-4 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human CTLA-4 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human B7-1 (hFc tag) with a linear range of 1-4 ng/mL.
Source HEK293-derived Human B7-1 protein Val35-Asn242 (Accession# P33681-1) with a human IGg1 Fc tag at the C-terminus.
Predicted Molecular Mass 49.9 kDa
SDS-PAGE 66-80 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • 12 months from the date of receipt, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B.

References:

1. Peach RJ. et al. (1995). J Biol Chem. 36: 21181-21187.
2. Vasilevko V. et al. (2002). DNA Cell Biol. 3: 137-149.
3. Short JJ. et al. (2006). Virus Res. 122:144-153.