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验证数据展示

  • WB analysis using 16988-1-AP

    WB analysis using 16988-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16988-1-AP (HSDL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 16988-1-AP

    IHC staining of human breast cancer using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 16988-1-AP

    IHC staining of human breast cancer using 16988-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16988-1-AP (HSDL1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSDL1 Polyclonal antibody

HSDL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16988-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

HSDL1, SDR12C3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, fetal human brain tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

16988-1-AP targets HSDL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag10588

Product name: Recombinant human HSDL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-330 aa of BC018084

Sequence: MAAVDSFYLLYREIARSCNCYMEALALVGAWYTARKSITVICDFYSLIRLHFIPRLGSRADLIKQYGRWAVVSGATDGIGKAYAEELASRGLNIILISRNEEKLQVVAKDIADTYKVETDIIVADFSSGREIYLPIREALKDKDVGILVNNVGVFYPYPQYFTQLSEDKLWDIINVNIAAASLMVHVVLPGMVERKKGAIVTISSGSCCKPTPQLAAFSASKAYLDHFSRALQYEYASKGIFVQSLIPFYVAASMTAPSNFLHRCSWLVPSPKVYAHHAVSTLGISKRTTGYWSHSIQFLFAQYMPEWLWVWGANILNRSLRKEALSCTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hydroxysteroid dehydrogenase like 1
别名 HSDL1, SDR12C3
计算分子量 330 aa, 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC018084
基因名称 HSDL1
Gene ID (NCBI) 83693
RRIDAB_2120235
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3SXM5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HSDL1 antibody 16988-1-APDownload protocol
IHC protocol for HSDL1 antibody 16988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy

Authors - Zhao-Xin Zhang
View Article
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
View Article