验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, EC109 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86974-2-RR targets HP1BP3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag18709 Product name: Recombinant human HP1BP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-302 aa of BC045660 Sequence: MASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | heterochromatin protein 1, binding protein 3 |
| 别名 | Heterochromatin protein 1-binding protein 3, HP1 BP74, Protein HP1 BP74, Protein HP1-BP74 |
| 计算分子量 | 553 aa, 61 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC045660 |
| 基因名称 | HP1BP3 |
| Gene ID (NCBI) | 50809 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q5SSJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HP1BP3 (heterochromatin protein 1-binding protein 3) is an evolutionarily conserved nuclear protein that functions as a non-canonical linker histone. It is broadly expressed in most vertebrate tissues and is structurally related to the histone H1 family, containing three globular domains and a highly basic C-terminal tail reminiscent of H1 proteins . Unique to HP1BP3 are an acidic N-terminal stretch and a consensus HP1-binding motif (PxVxL), through which it associates with heterochromatin protein 1 (HP1α/β) and is enriched at perinucleolar heterochromatin regions marked by H3K9me3. Functionally, HP1BP3 acts as a modulator of gene expression and genome stability. Alternative splicing was reported to produce 4 different isoforms of HP1BP3, the calculated MW corresponding to 61, 57, 44, 15 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HP1BP3 antibody 86974-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
