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验证数据展示

  • IHC staining of mouse testis using 10496-1-AP (same clone as 10496-1-PBS)

    IHC staining of mouse testis using 10496-1-AP (same clone as 10496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10496-1-AP (HOXD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10496-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 10496-1-AP (same clone as 10496-1-PBS)

    IF Staining of U2OS using 10496-1-AP (same clone as 10496-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HOXD3 antibody (10496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10496-1-PBS in a different storage buffer formulation.

HOXD3 Polyclonal antibody, PBS Only

HOXD3 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 10496-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10496-1-PBS targets HOXD3 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0768

Product name: Recombinant human HOXD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-200 aa of BC005124

Sequence: MLFEQGQQALELPECTMQKAAYYENPGLFGGYGYSKTTDTYGYSTPHQPYPPPAAASSLDTDYPGSACSIQSSAPLRAPAHKGAELNGSCMRPGTGNSQGGGGGSQPPGLNSEQQPPQPPPPPPTLPPSSPTNPGGGVPAKKPKGGPNASSSSATISKQIFPWMKESRQNSKQKNSCATAGESCEDKSPPGPASKRVRTA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox D3
别名
计算分子量 46 kDa
GenBank蛋白编号BC005124
基因名称 HOXD3
Gene ID (NCBI) 3232
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31249
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Homeobox D3 (HOXD3), a member of the Homeobox (Hox) family of master transcription factors, is a homeobox transcription factor that promotes angiogenesis and collagen synthesis. HOXD3 is expressed during normal embryogenesis, skin development, and fetal wound healing (PMID: 14633614). The overexpression of HOXD3 plays a critical role in breast cancer stemness and drug resistance via integrin β3-mediated Wnt/β-catenin signaling (PMID: 29698974).

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