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验证数据展示

  • WB analysis of HeLa using 20632-1-AP

    WB analysis of HeLa using 20632-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20632-1-AP (HOXC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20632-1-AP

    IF Staining of HeLa using 20632-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20632-1-AP (HOXC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HOXC10 Polyclonal antibody

HOXC10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20632-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20632-1-AP targets HOXC10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14620

Product name: Recombinant human HOXC10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC001293

Sequence: MTCPRNVTPNSYAEPLAAPGGGERYSRSAGMYMQSGSDFNCGVMRGCGLAPSLSKRDEGSSPSLALNTYPSYLSQLDSWGDPKAAYRLEQPVGRPLSSCSYPPSVKEENVCCMYSAEKRAKSGPEAALYSHPLPESCLGEHEVPVPSYYRASPSYSALDKTPHCSGANDFEAPFEQRASLNPRAEHLESPQLGGKVSFPETPKSDSQTPSPNEIKTEQSLAGPKGSPSESEKERAKAADSSPDTSDNEAKEEIKAENTTG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox C10
别名 HOX3I, Homeobox protein Hox-C10, Homeobox protein Hox-3I
计算分子量 342 aa, 38 kDa
观测分子量 50 kDa
GenBank蛋白编号BC001293
基因名称 HOXC10
Gene ID (NCBI) 3226
RRIDAB_10697666
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYD6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HOXC10, also named as Homeo box C10, is a 342 amino acid protein. The calculated molecular weight of HOXC10 is 38 kDa, but HOXC10 is detected about 50 kDa (PMID: 12853486)

实验方案

Product Specific Protocols
IF protocol for HOXC10 antibody 20632-1-APDownload protocol
WB protocol for HOXC10 antibody 20632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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