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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

    WB analysis of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10871-1-AP (HOOK1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10871-1-AP (same clone as 10871-1-PBS)

    IP experiment of mouse brain using 10871-1-AP (same clone as 10871-1-PBS)

    IP result of anti-HOOK1 (IP:10871-1-AP, 4ug; Detection:10871-1-AP 1:500) with mouse brain tissue lysate 4680ug. This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10871-1-AP (same clone as 10871-1-PBS)

    IHC staining of human colon cancer using 10871-1-AP (same clone as 10871-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10871-1-AP (HOOK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

    IF Staining of HEK-293 using 10871-1-AP (same clone as 10871-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using HOOK1 antibody (10871-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10871-1-PBS in a different storage buffer formulation.

HOOK1 Polyclonal antibody, PBS Only

HOOK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10871-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Protein Hook homolog 1, hook homolog 1 (Drosophila), HK1, h-hook1, hHK1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

10871-1-PBS targets HOOK1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HOOK1 fusion protein Ag1311 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hook homolog 1 (Drosophila)
别名 Protein Hook homolog 1, hook homolog 1 (Drosophila), HK1, h-hook1, hHK1
计算分子量 85 kDa
观测分子量 85 kDa
GenBank蛋白编号BC011621
基因名称 HOOK1
Gene ID (NCBI) 51361
RRIDAB_2120812
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJC3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS/Hook/FHIP complex which functions to promote vesicle trafficking and/or fusion via the HOPS (homotypic vesicular protein sorting) complex.