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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 17674-1-AP

    WB analysis using 17674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17674-1-AP (HNRNPH3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 17674-1-AP

    IF Staining of PC-3 using 17674-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using HNRNPH3 antibody (17674-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

HNRNPH3 Polyclonal antibody

HNRNPH3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17674-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

HNRPH3, hnRNP H3, hnRNP 2H9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, PC-3 cells, mouse testis tissue
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPH3 Ready-To-Use IHC Kit. HNRNPH3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17674-1-AP targets HNRNPH3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 HNRNPH3 fusion protein Ag11611 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein H3 (2H9)
别名 HNRPH3, hnRNP H3, hnRNP 2H9
计算分子量 331 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC039824
基因名称 HNRNPH3
Gene ID (NCBI) 3189
RRIDAB_2878425
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31942
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HNRNPH3 antibody 17674-1-APDownload protocol
IF protocol for HNRNPH3 antibody 17674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Dis Model Mech

Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.

Authors - Kyoung-In Cho
View Article