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HNRNPH3 Polyclonal antibody

HNRNPH3 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 17674-1-AP

Print datasheet

Synonyms

2H9, hnRNP 2H9, hnRNP H3, HNRNPH3, HNRPH3

验证数据展示

  • WB analysis using 17674-1-AP

    WB analysis using 17674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17674-1-AP (HNRNPH3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 17674-1-AP

    IF Staining of PC-3 using 17674-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using HNRNPH3 antibody (17674-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, PC-3 cells, mouse testis tissue
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17674-1-AP targets HNRNPH3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HNRNPH3 fusion protein Ag11611 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein H3 (2H9)
Synonyms 2H9, hnRNP 2H9, hnRNP H3, HNRNPH3, HNRPH3
Calculated Molecular Weight 331 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC039824
Gene Symbol HNRNPH3
Gene ID (NCBI) 3189
RRIDAB_2878425
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HNRNPH3 antibody 17674-1-APDownload protocol
IF protocol for HNRNPH3 antibody 17674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Dis Model Mech

Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes.

Authors - Kyoung-In Cho
View Article