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HMGA1 Polyclonal antibody

HMGA1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15398-1-AP

Print datasheet

Synonyms

high mobility group AT hook 1, High mobility group protein A1, High mobility group protein R, HMG I(Y), HMG R, HMGA1, HMGA1A, HMGIY

验证数据展示

  • WB analysis of COLO 320 using 15398-1-AP

    WB analysis of COLO 320 using 15398-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 15398-1-AP (HMGA1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15398-1-AP

    IHC staining of human prostate cancer using 15398-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15398-1-AP (HMGA1 Antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15398-1-AP targets HMGA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited ReactivityHamster
Immunogen HMGA1 fusion protein Ag7613 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name high mobility group AT-hook 1
Synonyms high mobility group AT hook 1, High mobility group protein A1, High mobility group protein R, HMG I(Y), HMG R, HMGA1, HMGA1A, HMGIY
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC004924
Gene Symbol HMGA1
Gene ID (NCBI) 3159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17096
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

发表文章

SpeciesApplicationTitle
HamsterWB

Lipids

The Safety and Anti-Hypercholesterolemic Effect of Coptisine in Syrian Golden Hamsters.

Authors - Kai He
View Article