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验证数据展示

  • IHC staining of human brain using 15283-1-AP (same clone as 15283-1-PBS)

    IHC staining of human brain using 15283-1-AP (same clone as 15283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15283-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 15283-1-AP (same clone as 15283-1-PBS)

    IHC staining of human brain using 15283-1-AP (same clone as 15283-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15283-1-AP (HJURP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15283-1-PBS in a different storage buffer formulation.

  • WB analysis using 15283-1-AP (same clone as 15283-1-PBS)

    WB analysis using 15283-1-AP (same clone as 15283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15283-1-AP (HJURP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15283-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

HJURP Polyclonal antibody, PBS Only

HJURP Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15283-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15283-1-PBS targets HJURP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7402

Product name: Recombinant human HJURP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 394-748 aa of BC001940

Sequence: ATYNLDEENRFRTLKWLISPVKIVSRPTIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSSKAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSKTLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPDPHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGASDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Holliday junction recognition protein
别名
计算分子量 86 kDa
观测分子量 84 kDa
GenBank蛋白编号BC001940
基因名称 HJURP
Gene ID (NCBI) 55355
RRIDAB_2116879
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NCD3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

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