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验证数据展示

  • IF Staining of HepG2 using 83162-7-RR (same clone as 83162-7-PBS)

    IF Staining of HepG2 using 83162-7-RR (same clone as 83162-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83162-7-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83162-7-RR (same clone as 83162-7-PBS)

    FC experiment of HepG2 using 83162-7-RR (same clone as 83162-7-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Hic2 Recombinant Antibody (83162-7-RR, Clone:230177E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83162-7-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 83162-7-RR (same clone as 83162-7-PBS)

    IF Staining of U2OS using 83162-7-RR (same clone as 83162-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIC2 antibody (83162-7-RR, Clone: 230177E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83162-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83162-7-RR

    Affinity and Kinetic Characterization of 83162-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83162-7-RR against Human HIC2 were performed. The affinity constant is 0.527 nM.

HIC2 Recombinant monoclonal antibody, PBS Only

HIC2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83162-7-PBS

产品说明书

CloneNo. 230177E8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83162-7-PBS targets HIC2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18789

Product name: Recombinant human HIC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 161-430 aa of BC094787

Sequence: RSQRLSTASVIQARYQGLVDGRKGAHAPQELPQAKGSDDELFLGGSNQDSVQGLGRAVCPAGGEAGLGGCSSSTNGSSGGCEQELGLDLSKKSPPLPPATPGPHLTPDDAAQLSDSQHGSPPAASAPPVANSASYSELGGTPDEPMDLEGAEDNHLSLLEAPGGQPRKSLRHSTRKKEWGKKEPVAGSPFERREAGPKGPCPGEEGEGVGDRVPNGILASGAGPSGPYGEPPYPCKEEEENGKDASEDSAQSGSEGGSGHASAHYMYRQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypermethylated in cancer 2
别名 Hic-3, Hic-2, HIC1-related gene on chromosome 22 protein, Hic 3, Hic 2
计算分子量 615 aa, 66 kDa
GenBank蛋白编号BC094787
基因名称 HIC2
Gene ID (NCBI) 23119
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96JB3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIC2 (hypermethylated in cancer 2) is a transcription factor related to the tumor suppressor HIC1, required for normal cardiac development and lost in distal variants of 22q11 Deletion Syndrome (PMID: 29061339). HIC2 is also a POK family protein, and the amino acid sequence of its BTB/POZ and zinc-finger domains are similar to those of HIC1(PMID: 31127867). Studies have shown that HIC2 plays a role in suppressing fetal genes for normal development of the heart and circulatory system, in embryogenesis (PMID: 24748541).

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