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  • KD/KO Validated

HDAC5-specific Polyclonal antibody

HDAC5-specific Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)


WB, IF/ICC, IHC, ELISA and More (1)



Cat No : 16166-1-AP

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Antigen NY CO 9, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9


Positive WB detected inHeLa cells, HEK-293 cells, fetal human brain tissue
Positive IHC detected inmouse brain tissue, human brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HDAC5 Ready-To-Use IHC Kit. HDAC5 primary antibody included.


Western Blot (WB)WB : 1:100-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


16166-1-AP targets HDAC5-specific in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone deacetylase 5
Synonyms Antigen NY CO 9, HD5, HDAC5, histone deacetylase 5, KIAA0600, NY CO 9
Calculated Molecular Weight 122 kDa
Observed Molecular Weight120-140 kDa
GenBank Accession NumberBC051824
Gene Symbol HDAC5
Gene ID (NCBI) 10014
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


Histone acetylation and deacetylation alternately expose and occlude DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3, and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression, and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.


Product Specific Protocols
WB protocol for HDAC5-specific antibody 16166-1-APDownload protocol
IHC protocol for HDAC5-specific antibody 16166-1-APDownload protocol
IF protocol for HDAC5-specific antibody 16166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Authors - Xun Huang
mouse, ratWB,IHC,IF

Kidney Int

Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy.

Authors - Xiaojie Wang

Cancer Lett

ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer

Authors - Chen Zhou

J Cell Mol Med

Low levels of AMPK promote epithelial-mesenchymal transition in lung cancer primarily through HDAC4- and HDAC5-mediated metabolic reprogramming.

Authors - Shoujie Feng
  • KD Validated

J Cell Mol Med

HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury.

Authors - He Min M

Biomed Pharmacother

Trichostatin A increases BDNF protein expression by improving XBP-1s/ATF6/GRP78 axis in Schwann cells of diabetic peripheral neuropathy.

Authors - Jiahui An
  • KD Validated