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验证数据展示

  • WB analysis of HL-60 using 13588-1-AP (same clone as 13588-1-PBS)

    WB analysis of HL-60 using 13588-1-AP (same clone as 13588-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13588-1-AP (GZMB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

    IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

    IHC staining of human lymphoma using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13588-1-AP (GZMB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    IF Staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Granzyme B antibody (13588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    IF Staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using Granzyme B antibody (13588-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13588-1-AP (Granzyme B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    IHC staining of mouse spleen using 13588-1-AP (same clone as 13588-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13588-1-AP (Granzyme B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 13588-1-AP (same clone as 13588-1-PBS)

    IF Staining of Jurkat using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Granzyme B antibody (13588-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 13588-1-AP (same clone as 13588-1-PBS)

    IF Staining of MCF-7 using 13588-1-AP (same clone as 13588-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Granzyme B antibody (13588-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13588-1-PBS in a different storage buffer formulation.

Granzyme B Polyclonal antibody, PBS Only

Granzyme B Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 13588-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13588-1-PBS targets Granzyme B in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3883

Product name: Recombinant human GZMB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-247 aa of BC030195

Sequence: MQPILLLLAFLLLPRADAGEIIGGHEAKPHSRPYMAYLMIWDQKSLKRCGGFLIQDDFVLTAAHCWGSSINVTLGAHNIKEQEPTQQFIPVKRPIPHPAYNPKNFSNDIMLLQLERKAKRTRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSSFVHWIKKTMKRH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
别名 GZMB, Cathepsin G-like 1, CCPI, CGL 1, CGL1
计算分子量 247 aa, 28 kDa
观测分子量 33 kDa
GenBank蛋白编号BC030195
基因名称 Granzyme B
Gene ID (NCBI) 3002
RRIDAB_2114429
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10144
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.

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