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验证数据展示

  • WB analysis using 12412-1-AP (same clone as 12412-1-PBS)

    WB analysis using 12412-1-AP (same clone as 12412-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

    IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

    IF Staining of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GSTM1 Polyclonal antibody, PBS Only

GSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12412-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

EC:2.5.1.18, glutathione S transferase mu 1, Glutathione S-transferase Mu 1, GST class mu 1, GST class-mu 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12412-1-PBS targets GSTM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3113

Product name: Recombinant human GSTM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-181 aa of BC024005

Sequence: MPMILGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQTMDNHMQLGMICYNPEFEKLKPKYLEELPEKLKLYSEFLGKRPWFAGNKGLEKISAYMKSSRFLPRPVFSKMAVWGNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutathione S-transferase mu 1
别名 EC:2.5.1.18, glutathione S transferase mu 1, Glutathione S-transferase Mu 1, GST class mu 1, GST class-mu 1
计算分子量 181 aa, 21 kDa
观测分子量 27 kDa
GenBank蛋白编号BC024005
基因名称 GSTM1
Gene ID (NCBI) 2944
RRIDAB_2115925
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09488
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSTM1 belongs to the superfamily of glutathione-Stransferases (GSTs) of phase 2 enzymes that catalyze a number of distinct glutathione-dependent reactions. GSTM1 gene has been a natural candidate in studies of susceptibility to inflammatory airway diseases with environmental components because of the high prevalence of the null polymorphism and its role in detoxification (PMID: 22683820).

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