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验证数据展示

  • WB analysis using 20146-1-AP (same clone as 20146-1-PBS)

    WB analysis using 20146-1-AP (same clone as 20146-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20146-1-AP (GPR81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    IHC staining of mouse pancreas using 20146-1-AP (same clone as 20146-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20146-1-AP (GPR81 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20146-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GPR81 Polyclonal antibody, PBS Only

GPR81 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 20146-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

FKSG80, G protein coupled receptor 104, G protein coupled receptor 81, GPR104, G-protein coupled receptor 104

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

20146-1-PBS targets GPR81 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13682

Product name: Recombinant human GPR81 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 250-346 aa of BC066881

Sequence: SACDPSVHGALHITLSFTYMNSMLDPLVYYFSSPSFPKFYNKLKICSLKPKQPGHSKTQRPEEMPISNLGRRSCISVANSFQSQSDGQWDPHIVEWH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 81
别名 FKSG80, G protein coupled receptor 104, G protein coupled receptor 81, GPR104, G-protein coupled receptor 104
计算分子量 346 aa, 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC066881
基因名称 GPR81
Gene ID (NCBI) 27198
RRIDAB_2878647
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXC0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPR81 (G protein-coupled receptor 81) is one of a large family of GPRs with low affinity for hydroxy-carboxylic acid structure ligands (PMID: 24657625). Lactate functions as a signaling molecule by serving as an agonist for the GPR81, involving both autocrine and paracrine mechanisms (PMID: 31836453). Moreover, A higher molecular mass band (∼60 kDa) is present in the brain and adipose tissue. This band is also strong in the transfected HeLa cells but weak in native HeLa cells and is therefore probably a form of GPR81, possibly a glycosylated form of the receptor (PMID: 23696276).