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验证数据展示

  • WB analysis using 14074-1-AP

    WB analysis using 14074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14074-1-AP (GNPTG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14074-1-AP

    WB analysis using 14074-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14074-1-AP (GNPTG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GNPTG Polyclonal antibody

GNPTG Polyclonal Antibody for WB, ELISA
Cat No. 14074-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

N-acetylglucosamine-1-phosphotransferase subunit gamma, LP2537, GNPTAG, GlcNAc-1-phosphotransferase subunit gamma, CAB56184

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14074-1-AP targets GNPTG in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5153

Product name: Recombinant human GNPTG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 5-304 aa of BC014592

Sequence: LARLLLLLGLSAGGPAPAGAAKMKVVEEPNAFGVNNPFLPQASRLQAKRDPSPVSGPVHLFRLSGKCFSLVESTYKYEFCPFHNVTQHEQTFRWNAYSGILGIWHEWEIANNTFTGMWMRDGDACRSRSRQSKVELACGKSNRLAHVSEPSTCVYALTFETPLVCHPHALLVYPTLPEALQRQWDQVEQDLADELITPQGHEKLLRTLFEDAGYLKTPENEPTQLEGGPDSLGFETLENCRKAHKELSKEIKRLKGLLTQHGIPYTRPTETSNLEHLGHETPRAKSPEQLRGDPGLRGSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 N-acetylglucosamine-1-phosphate transferase, gamma subunit
别名 N-acetylglucosamine-1-phosphotransferase subunit gamma, LP2537, GNPTAG, GlcNAc-1-phosphotransferase subunit gamma, CAB56184
计算分子量 34 kDa
观测分子量34 kDa, 68~70 kDa
GenBank蛋白编号BC014592
基因名称 GNPTG
Gene ID (NCBI) 84572
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJJ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GlcNAc-phosphotransferase is a multisubunit enzyme with an α2β2γ2 arrangement that requires a detergent for solubilization. Recent cloning of cDNAs and genes encoding these subunits revealed that the α- and β-subunits are encoded by a single gene as a precursor, whereas the γ-subunit is encoded by a second gene. Sugar composition and/ or additional modifications which alter the charge of the GNPTAG oligosaccharide chains are variable in a cell type-depending manner.

实验方案

Product Specific Protocols
WB protocol for GNPTG antibody 14074-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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