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验证数据展示

  • WB analysis using 11254-1-AP (same clone as 11254-1-PBS)

    WB analysis using 11254-1-AP (same clone as 11254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11254-1-AP (GLRX3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11254-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 11254-1-AP (same clone as 11254-1-PBS)

    IHC staining of human stomach cancer using 11254-1-AP (same clone as 11254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11254-1-AP (GLRX3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11254-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GLRX3 Polyclonal antibody, PBS Only

GLRX3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11254-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Glutaredoxin 3, GLRX4, Glutaredoxin-3, GRX3, GRX4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

11254-1-PBS targets GLRX3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1775

Product name: Recombinant human GLRX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-289 aa of BC014372

Sequence: AQMNEVMAELAKELPQVSFVKLEAEGVPEVSEKYEISSVPTFLFFKNSQKIDRLDGAHAPELTKKVQRHASSGSFLSSANEHLKEDLNLRLKKLTHAAPCMLFMKGTPQEPRCGFSKQMVEILHKHNIQFSSFDIFSDEEVRQGLKAYSSWPTYPQLYVSGELIGGLDIIKELEASEELDTICPKAPKLEERLKVLTNKASVMLFMKGNKQEAKCGFSKQILEILNSTGVEYETFDILEDEEVRQGLKAYSNWPTYPQLYVKGELVGGLDIVKELKENGELLPILRGEN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glutaredoxin 3
别名 Glutaredoxin 3, GLRX4, Glutaredoxin-3, GRX3, GRX4
计算分子量 37 kDa
观测分子量 37-40 kDa
GenBank蛋白编号BC014372
基因名称 GLRX3
Gene ID (NCBI) 10539
RRIDAB_2110374
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO76003
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GLRX3(Glutaredoxin-3) is also named as PICOT, TXNL2. Mammalian glutaredoxin 3 is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals(PMID:20226171). Glrx3 could take a pivotal role in colon and lung cancer cells during the tumorigenesis, which is expressed in lung and colon cancers consistently and preferentially(PMID:19797004).

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