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验证数据展示

  • WB analysis of human testis using 83584-3-RR

    WB analysis of human testis using 83584-3-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83584-3-RR (GLIPR1L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-3 using 83584-3-RR

    FC experiment of PC-3 using 83584-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug GLIPR1L1 Recombinant antibody (83584-3-RR, Clone:240594D10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83584-3-RR

    Affinity and Kinetic Characterization of 83584-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83584-3-RR against Human GLIPR1L1 were performed. The affinity constant is 6.77 nM.

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GLIPR1L1 Recombinant monoclonal antibody

GLIPR1L1 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83584-3-RR

产品说明书

CloneNo. 240594D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83584-3-RR targets GLIPR1L1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14852

Product name: Recombinant human GLIPR1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-233 aa of BC014603

Sequence: SKIPSITDPHFIDNCIEAHNEWRGKVNPPAADMKYMIWDKGLAKMAKAWANQCKFEHNDCLDKSYKCYAAFEYVGENIWLGGIKSFTPRHAITAWYNETQFYDFDSLSCSRVCGHYTQLVWANSFYVGCAVAMCPNLGGASTAIFVCNYGPAGNFANMPPYVRGESCSLCSKEEKCVKNLCKNPFLKPTGRAPQQTAFNPFSLGFLLLRIF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GLI pathogenesis-related 1 like 1
别名 UNQ2972/PRO7434, PRO7434, GLIPR1-like protein 1, GLIPR1 like protein 1, ALKN2972
计算分子量 242 aa, 27 kDa
观测分子量25 kDa
GenBank蛋白编号BC014603
基因名称 GLIPR1L1
Gene ID (NCBI) 256710
RRIDAB_3671198
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ6UWM5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLIPR1L1 antibody 83584-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols