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验证数据展示

  • IHC staining of human heart using 25379-1-AP

    IHC staining of human heart using 25379-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25379-1-AP (GFRA3 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 25379-1-AP

    IHC staining of human heart using 25379-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25379-1-AP (GFRA3 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of mouse heart using 25379-1-AP

    WB analysis of mouse heart using 25379-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25379-1-AP (GFRA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

GFRA3 Polyclonal antibody

GFRA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25379-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GDNF family receptor alpha 3, GDNF receptor alpha 3, GDNFR alpha 3, GFR alpha 3, GFRA3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25379-1-AP targets GFRA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GFRA3 fusion protein Ag13253 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GDNF family receptor alpha 3
别名 GDNF family receptor alpha 3, GDNF receptor alpha 3, GDNFR alpha 3, GFR alpha 3, GFRA3
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC037951
基因名称 GFRA3
Gene ID (NCBI) 2676
RRIDAB_2880050
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60609
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GFRA3 is a receptor for the glial cell line-derived neurotrophic factor, ARTN (artemin). It mediates the artemin-induced autophosphorylation and activation of the RET receptor tyrosine kinase. GFRA3 has two isoforms with MW 45 kDa and 41 kDa. It can be cleaved to be a mature form with MW 41 kDa or 37 kDa.

实验方案

Product Specific Protocols
WB protocol for GFRA3 antibody 25379-1-APDownload protocol
IHC protocol for GFRA3 antibody 25379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols