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验证数据展示

  • WB analysis using 68083-1-Ig

    WB analysis using 68083-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68083-1-Ig (GDAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of mouse brain using 68083-1-Ig

    IF Staining of mouse brain using 68083-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of rat brain using 68083-1-Ig

    IHC staining of rat brain using 68083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 68083-1-Ig

    IHC staining of rat brain using 68083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 68083-1-Ig

    IF Staining of mouse brain using 68083-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

GDAP1 Monoclonal antibody

GDAP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 68083-1-Ig

产品说明书

CloneNo. 3E10D4

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat, rabbit, pig

应用

WB, IHC, IF-P, ELISA

别名

CMTRIA, GDAP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inpig brain tissue, Rabbit brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68083-1-Ig targets GDAP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit, pig
免疫原

CatNo: Ag28616

Product name: Recombinant human GDAP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-257 aa of BC024939

Sequence: MRLNSTGEVPVLIHGENIICEATQIIDYLEQTFLDERTPRLMPDKESMYYPRVQHYRELLDSLPMDAYTHGCILHPELTVDSMIPAYATTRIRSQIGNTESELKKLAEENPDLQEAYIAKQKRLKSKLLDHDNVKYLKKILDELEKVLDQVETELQRRNEETPEEGQQPWLCGESFTLADVSLAVTLHRLKFLGFARRNWGNGKRPNLETYYERVLKRKTFNKVLGHVNNILISAVLPTAFRVAKKRAPKVLGTTLV

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 ganglioside-induced differentiation-associated protein 1
别名 CMTRIA, GDAP1
计算分子量 358 aa, 41 kDa
观测分子量38-40 kDa
GenBank蛋白编号BC024939
基因名称 GDAP1
Gene ID (NCBI) 54332
RRIDAB_2918820
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8TB36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.

实验方案

Product Specific Protocols
IF protocol for GDAP1 antibody 68083-1-IgDownload protocol
IHC protocol for GDAP1 antibody 68083-1-IgDownload protocol
WB protocol for GDAP1 antibody 68083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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