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GDAP1 Monoclonal antibody

GDAP1 Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3E10D4

Cat No : 68083-1-Ig

Print datasheet

Synonyms

CMTRIA, GDAP1

验证数据展示

  • WB analysis using 68083-1-Ig

    WB analysis using 68083-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68083-1-Ig (GDAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • IF Staining of mouse brain using 68083-1-Ig

    IF Staining of mouse brain using 68083-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of rat brain using 68083-1-Ig

    IHC staining of rat brain using 68083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 68083-1-Ig

    IHC staining of rat brain using 68083-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68083-1-Ig (GDAP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 68083-1-Ig

    IF Staining of mouse brain using 68083-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using GDAP1 antibody (68083-1-Ig, Clone: 3E10D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig brain tissue, Rabbit brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68083-1-Ig targets GDAP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen GDAP1 fusion protein Ag28616 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ganglioside-induced differentiation-associated protein 1
Synonyms CMTRIA, GDAP1
Calculated Molecular Weight 358 aa, 41 kDa
Observed Molecular Weight38-40 kDa
GenBank Accession NumberBC024939
Gene Symbol GDAP1
Gene ID (NCBI) 54332
RRIDAB_2918820
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TB36
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GDAP1 is a member of the ganglioside-induced differentiation-associated protein family, which may play a role in a signal transduction pathway during neuronal development. Mutations in this gene have been associated with various forms of Charcot-Marie-Tooth Disease and neuropathy.

实验方案

Product Specific Protocols
WB protocol for GDAP1 antibody 68083-1-IgDownload protocol
IHC protocol for GDAP1 antibody 68083-1-IgDownload protocol
IF protocol for GDAP1 antibody 68083-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols