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验证数据展示

  • WB analysis using 17859-1-AP (same clone as 17859-1-PBS)

    WB analysis using 17859-1-AP (same clone as 17859-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17859-1-AP (GCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 17859-1-AP (same clone as 17859-1-PBS)

    WB analysis of COLO 320 using 17859-1-AP (same clone as 17859-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17859-1-AP (GCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    IHC staining of human small intestine using 17859-1-AP (same clone as 17859-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17859-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GCS1 Polyclonal antibody, PBS Only

GCS1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17859-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

17859-1-PBS targets GCS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12321

Product name: Recombinant human MOGS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 492-837 aa of BC028337

Sequence: DEARARVPPEFLVQRAVHANPPTLLLPVAHMLEVGDPDDLAFLRKALPRLHAWFSWLHQSQAGPLPLSYRWRGRDPALPTLLNPKTLPSGLDDYPRASHPSVTERHLDLRCWVALGARVLTRLAEHLGEAEVAAELGPLAASLEAAESLDELHWAPELGVFADFGNHTKAVQLKPRPPQGLVRVVGRPQPQLQYVDALGYVSLFPLLLRLLDPTSSRLGPLLDILADSRHLWSPFGLRSLAASSSFYGQRNSEHDPPYWRGAVWLNVNYLALGALHHYGHLEGPHQARAAKLHGELRANVVGNVWRQYQATGFLWEQYSDRDGRGMGCRPFHGWTSLVLLAMAEDY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mannosyl-oligosaccharide glucosidase
别名
计算分子量 837 aa, 92 kDa
观测分子量 80-92 kDa
GenBank蛋白编号BC028337
基因名称 GCS1
Gene ID (NCBI) 7841
RRIDAB_10694270
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13724
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

MOGS (Mannosyl-oligosaccharide glucosidase), also known as GCS1, is the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor (PMID: 38187061). The resulting Glc(2)-Man(9)-GlcNAc(2) intermediate is then further modified by glucosidase II and several ER- and Golgi-resident mannosidases and glycosyltransferases, finally yielding a complex array of glycan structures (PMID: 3896128). MOGS has two isoforms, and it is located in the lumen of the endoplasmic reticulum.

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