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验证数据展示

  • WB analysis using 17845-1-AP

    WB analysis using 17845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17845-1-AP (GCNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 17845-1-AP

    WB analysis using 17845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17845-1-AP (GCNT1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GCNT1 Polyclonal antibody

GCNT1 Polyclonal Antibody for WB, ELISA
Cat No. 17845-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase, C2GlcNAcT, C2GNT1, C2GNT-L, Core 2 beta-1,6-N-acetylglucosaminyltransferase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTT cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17845-1-AP targets GCNT1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag12264

Product name: Recombinant human GCNT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 41-428 aa of BC074885

Sequence: VSVRHLELAGENPSSDINCTKVLQGDVNEIQKVKLEILTVKFKKRPRWTPDDYINMTSDCSSFIKRRKYIVEPLSKEEAEFPIAYSIVVHHKIEMLDRLLRAIYMPQNFYCIHVDTKSEDSYLAAVMGIASCFSNVFVASRLESVVYASWSRVQADLNCMKDLYAMSANWKYLINLCGMDFPIKTNLEIVRKLKLLMGENNLETERMPSHKEERWKKRYEVVNGKLTNTGTVKMLPPLETPLFSGSAYFVVSREYVGYVLQNEKIQKLMEWAQDTYSPDEYLWATIQRIPEVPGSLPASHKYDLSDMQAVARFVKWQYFEGDVSKGAPYPPCDGVHVRSVCIFGAGDLNWMLRKHHLFANKFDVDVDLFAIQCLDEHLRHKALETLKH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucosaminyl (N-acetyl) transferase 1, core 2 (beta-1,6-N-acetylglucosaminyltransferase)
别名 Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase, C2GlcNAcT, C2GNT1, C2GNT-L, Core 2 beta-1,6-N-acetylglucosaminyltransferase
计算分子量 428 aa, 50 kDa
观测分子量40-50 kDa
GenBank蛋白编号BC074885
基因名称 GCNT1
Gene ID (NCBI) 2650
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02742
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glucosaminyl (N-acetyl) transferase 1 (GCNT1, also known as C2GNT1), is a glycosyltransferase enzyme that plays an essential role in the formation of core 2 branched O-glycans that is crucial to the final definition of O-glycan structure (PMID: 37813880). GCNT1 promotes the growth of prostate tumors and regulates oncogenic gene expression pathways that are important for disease progression (PMID: 24854630).

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