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验证数据展示

  • WB analysis using 10917-2-AP

    WB analysis using 10917-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 10917-2-AP

    IP experiment of K-562 using 10917-2-AP

    IP result of anti-GATA1 (IP:10917-2-AP, 4ug; Detection:10917-2-AP 1:500) with K-562 cells lysate 4000ug.

  • IHC staining of mouse testis using 10917-2-AP

    IHC staining of mouse testis using 10917-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of K-562 using 10917-2-AP

    WB analysis of K-562 using 10917-2-AP

    WB result of GATA1 antibody (10917-2-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA1 transfected K-562 cells.

  • Featured Product
  • KD/KO Validated

GATA1 Polyclonal antibody

GATA1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10917-2-AP

产品说明书

引用文献(19)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, IHC, ELISA and More (3)

别名

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, Raji cells, K-562 cells, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10917-2-AP targets GATA1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP, ChIP
经测试反应性 human, mouse
文献引用反应性human, mouse, zebrafish
免疫原

CatNo: Ag1350

Product name: Recombinant human GATA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-316 aa of BC009797

Sequence: MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHHQHYCGGSAQLMRAQSMASRGGVVSFS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GATA binding protein 1 (globin transcription factor 1)
别名 ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT
计算分子量 43 kDa
观测分子量 50-55 kDa, 40-45 kDa
GenBank蛋白编号BC009797
基因名称 GATA1
Gene ID (NCBI) 2623
RRIDAB_2108279
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15976
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757]. GATA1 exists two isoform with the molecular weight 50-55 kDa and 40-45 kDa (PMID: 31189107 ).

实验方案

Product Specific Protocols
WB protocol for GATA1 antibody 10917-2-APDownload protocol
IP protocol for GATA1 antibody 10917-2-APDownload protocol
IHC protocol for GATA1 antibody 10917-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC,IF,ChIP

Theranostics

Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

Authors - Soo Hyun Kang
  • KD Validated
View Article
zebrafishWB

Adv Sci (Weinh)

Nap1L4a Cooperates with Scl/Klf1 to Recruit H2A.Z in Mediating Interactions Among Cis-Regulatory Elements and Transcription Required for Primitive Erythropoiesis in Zebrafish.

Authors - JiaHao Shi
View Article
mouseIHC

Biomater Res

Biomimetic Nanotechnology Overcoming the Blood-Testis Barrier for Testicular Protection in Chemotherapy.

Authors - Chaoli An
View Article
humanWB,IHC

Oncogene

microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.

Authors - R Su
View Article
humanWB

Phytother Res

Harmine promotes megakaryocyte differentiation and thrombopoiesis by activating the Rac1/Cdc42/JNK pathway through a potential target of 5-HTR2A

Authors - Xiaoxi Liu
View Article
humanWB

Biomed Pharmacother

Xanthotoxin, a novel inducer of platelet formation, promotes thrombocytopoiesis via IL-1R1 and MEK/ERK signaling

Authors - Jia Lai
View Article
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