验证数据展示

WB analysis using 10917-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10917-2-AP (GATA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 10917-2-AP
IP result of anti-GATA1 (IP:10917-2-AP, 4ug; Detection:10917-2-AP 1:500) with K-562 cells lysate 4000ug.
IHC staining of mouse testis using 10917-2-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10917-2-AP (GATA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
WB analysis of K-562 using 10917-2-AP
WB result of GATA1 antibody (10917-2-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA1 transfected K-562 cells.

Featured Product
KD/KO验证

GATA1 Polyclonal antibody

GATA1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10917-2-AP

产品说明书

引用文献(13)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IP, IHC, ELISA and More (3)

别名

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (13)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HL-60 cells, Raji cells, K-562 cells, NIH/3T3 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

应用	推荐稀释比
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 12 publications below
IHC	See 5 publications below
IF	See 1 publications below
CoIP	See 1 publications below
ChIP	See 2 publications below

产品信息

10917-2-AP targets GATA1 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IP, IHC, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP, ChIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, zebrafish
免疫原	CatNo: Ag1350 Product name: Recombinant human GATA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-316 aa of BC009797 Sequence: MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHHQHYCGGSAQLMRAQSMASRGGVVSFS 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	GATA binding protein 1 (globin transcription factor 1)
别名	ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT
计算分子量	43 kDa
观测分子量	50-55 kDa, 40-45 kDa
GenBank蛋白编号	BC009797
基因名称	GATA1
Gene ID (NCBI)	2623
RRID	AB_2108279
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P15976
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757]. GATA1 exists two isoform with the molecular weight 50-55 kDa and 40-45 kDa (PMID: 31189107 ).

实验方案

Product Specific Protocols
IHC protocol for GATA1 antibody 10917-2-AP	Download protocol
IP protocol for GATA1 antibody 10917-2-AP	Download protocol
WB protocol for GATA1 antibody 10917-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human,mouse	WB,IHC,IF,ChIP	Theranostics Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p Authors - Soo Hyun Kang KD Validated View Article
human	WB,IHC	Oncogene microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia. Authors - R Su View Article
mouse	WB,CoIP,ChIP	iScience Superenhancer-driven circRNA Myst4 involves in pulmonary artery smooth muscle cell ferroptosis in pulmonary hypertension Authors - Siyu He View Article
human	WB	Front Immunol FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome. Authors - Chunlin Wang View Article
mouse	IHC	Genes (Basel) Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector. Authors - Takehito Sugasawa View Article
human	WB,IHC	Oncotarget The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer. Authors - Yanan Zhang View Article

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验证数据展示

WB analysis using 10917-2-AP

IP experiment of K-562 using 10917-2-AP

IHC staining of mouse testis using 10917-2-AP

WB analysis of K-562 using 10917-2-AP

GATA1 Polyclonal antibody

GATA1 Polyclonal Antibody for WB, IP, IHC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

microRNA-23a, -27a and -24 synergistically regulate JAK1/Stat3 cascade and serve as novel therapeutic targets in human acute erythroid leukemia.

Superenhancer-driven circRNA Myst4 involves in pulmonary artery smooth muscle cell ferroptosis in pulmonary hypertension

FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome.

Proof of Gene Doping in a Mouse Model with a Human Erythropoietin Gene Transferred Using an Adenoviral Vector.

The transcription factor GATA1 and the histone methyltransferase SET7 interact to promote VEGF-mediated angiogenesis and tumor growth and predict clinical outcome of breast cancer.