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验证数据展示

  • WB analysis using 29272-1-AP (same clone as 29272-1-PBS)

    WB analysis using 29272-1-AP (same clone as 29272-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 29272-1-AP (GAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

    IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 29272-1-AP (same clone as 29272-1-PBS)

    WB analysis of K-562 using 29272-1-AP (same clone as 29272-1-PBS)

    WB result of GAB2 antibody (29272-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAB2 transfected K-562 cells. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GAB2 Polyclonal antibody, PBS Only

GAB2 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29272-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

29272-1-PBS targets GAB2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30347

Product name: Recombinant human GAB2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 588-676 aa of BC131711

Sequence: QNPVSASPVPSGTNSPAPKKSTGSVDYLALDFQPSSPSPHRKPSTSSVTSDEKVDYVQVDKEKTQALQNTMQEWTDVRQSSEPSKGAKL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRB2-associated binding protein 2
别名
计算分子量 676 aa, 74 kDa
观测分子量 74-90 kDa
GenBank蛋白编号BC131711
基因名称 GAB2
Gene ID (NCBI) 9846
RRIDAB_2918265
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UQC2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Growth factor receptor-bound protein 2 (GAB2), an adaptor protein, belongs to the Gab family and is involved in various biologic processes. Gab2 mainly mediates phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated kinase (ERK) signaling pathways. Furthermore, Gab2 governs tumorigenic signaling and is a novel potential therapeutic target in leukemia, breast cancer, ovarian cancer, and melanoma. Gab2 is the major 98-kDa phosphoprotein associated with SHP-2, PI 3-kinase, and Grb2 in normal human lymphocytes. (PMID: 32269703, PMID: 28842424, PMID: 28420432, PMID: 10849428)