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验证数据展示

  • WB analysis using 29084-1-AP (same clone as 29084-1-PBS)

    WB analysis using 29084-1-AP (same clone as 29084-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29084-1-AP (G6PC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse liver using 29084-1-AP (same clone as 29084-1-PBS)

    IF Staining of mouse liver using 29084-1-AP (same clone as 29084-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using G6PC antibody (29084-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29084-1-PBS in a different storage buffer formulation.

G6PC Polyclonal antibody, PBS Only

G6PC Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 29084-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, Indirect ELISA

别名

G6PC1, EC:3.1.3.9, G6Pase, G-6-Pase, G6Pase-alpha

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

29084-1-PBS targets G6PC in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag30614

Product name: Recombinant human G6PC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-121 aa of BC130478

Sequence: NLVFKWILFGQRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGSPSGHAM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucose-6-phosphatase, catalytic subunit
别名 G6PC1, EC:3.1.3.9, G6Pase, G-6-Pase, G6Pase-alpha
计算分子量 357 aa, 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC130478
基因名称 G6PC
Gene ID (NCBI) 2538
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35575
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glucose-6-phosphatase-α (G6PC) is a key enzyme in glucose homeostasis that catalyzes the hydrolysis of glucose-6-phosphate to glucose and phosphate in the terminal step of gluconeogenesis and glycogenolysis. G6PC activity is restricted to the liver , the kidney cortex and the small intestine and confers on these three organs the capacity to release glucose into the systemic circulation (PMID: 21983240).The encoded enzyme is anchored to the ER by nine transmembrane helices with the amino (N)-terminus in the lumen and the carboxyl (C)-terminus in the cytoplasm (PMID: 15542400).

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