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验证数据展示

  • WB analysis using 13057-2-AP

    WB analysis using 13057-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13057-2-AP

    IF Staining of HeLa using 13057-2-AP

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (13057-2-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis of HEK-293 using 13057-2-AP

    WB analysis of HEK-293 using 13057-2-AP

    WB result of G3BP1 antibody (13057-2-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-G3BP1 transfected HEK-293 cells.

  • IHC staining of human lung cancer using 13057-2-AP

    IHC staining of human lung cancer using 13057-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with G3BP1 antibody included
  • IP experiment of HEK-293 using 13057-2-AP

    IP experiment of HEK-293 using 13057-2-AP

    IP result of anti-G3BP1 (IP:13057-2-AP, 4ug; Detection:13057-2-AP 1:1000) with HEK-293 cells lysate 1040 ug.

  • IHC staining of human lung cancer using 13057-2-AP

    IHC staining of human lung cancer using 13057-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with G3BP1 antibody included
  • FC experiment of HeLa using 13057-2-AP

    FC experiment of HeLa using 13057-2-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug G3BP1 Polyclonal antibody (13057-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO验证

G3BP1 Polyclonal antibody

G3BP1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13057-2-AP

产品说明书

引用文献(216)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (5)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (3)

别名

ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1, G3BP-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inC6 cells, HEK-293 cells, human brain tissue, Neuro-2a cells, Jurkat cells, MCF-7 cells, HeLa cells, mouse kidney tissue, rat kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected insodium arsenite treated HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13057-2-AP targets G3BP1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey, chicken, zebrafish, drosophila melanogaster (fruit fly)
免疫原

CatNo: Ag3728

Product name: Recombinant human G3BP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 167-466 aa of BC006997

Sequence: PDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPIMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGVGRGLAPRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTPase activating protein (SH3 domain) binding protein 1
别名 ATP-dependent DNA helicase VIII, EC:3.6.4.12, G3BP, G3BP 1, G3BP-1
计算分子量 466 aa, 52 kDa
观测分子量68 kDa
GenBank蛋白编号BC006997
基因名称 G3BP1
Gene ID (NCBI) 10146
RRIDAB_2232034
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13283
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.

实验方案

Product Specific Protocols
FC protocol for G3BP1 antibody 13057-2-APDownload protocol
IF protocol for G3BP1 antibody 13057-2-APDownload protocol
IHC protocol for G3BP1 antibody 13057-2-APDownload protocol
IP protocol for G3BP1 antibody 13057-2-APDownload protocol
WB protocol for G3BP1 antibody 13057-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,human,chickenIF,CoIP

Cell

Diverse CMT2 neuropathies are linked to aberrant G3BP interactions in stress granules

Authors - Qinqin Cui
View Article
humanIF

Nature

m6A enhances the phase separation potential of mRNA.

Authors - Ryan J Ries
View Article
humanIP,IF

Science

Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.

Authors - Youngdae Gwon
View Article
humanIF

Cell

ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids.

Authors - Kathryn R Bowles
View Article
humanIF

Cell

RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether.

Authors - Ya-Cheng Liao
View Article
humanIF

Cell

Phase Separation of FUS Is Suppressed by Its Nuclear Import Receptor and Arginine Methylation.

Authors - Mario Hofweber
View Article
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