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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 27729-1-AP (same clone as 27729-1-PBS)

    WB analysis using 27729-1-AP (same clone as 27729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

  • WB analysis using 27729-1-AP (same clone as 27729-1-PBS)

    WB analysis using 27729-1-AP (same clone as 27729-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27729-1-AP (Flightless I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 27729-1-AP (same clone as 27729-1-PBS)

    IHC staining of human colon using 27729-1-AP (same clone as 27729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Flightless I antibody included
  • IHC staining of human colon using 27729-1-AP (same clone as 27729-1-PBS)

    IHC staining of human colon using 27729-1-AP (same clone as 27729-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27729-1-AP (Flightless I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Flightless I antibody included
  • IF Staining of A431 using 27729-1-AP (same clone as 27729-1-PBS)

    IF Staining of A431 using 27729-1-AP (same clone as 27729-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Flightless I antibody (27729-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27729-1-PBS in a different storage buffer formulation.

Flightless I Polyclonal antibody, PBS Only

Flightless I Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27729-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Flightless I Ready-To-Use IHC Kit. Flightless I primary antibody included.

产品信息

27729-1-PBS targets Flightless I in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Flightless I fusion protein Ag26865 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 flightless I homolog (Drosophila)
别名
计算分子量 1269 aa, 145 kDa
观测分子量 145-150 kDa
GenBank蛋白编号BC025300
基因名称 Flightless I
Gene ID (NCBI) 2314
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13045
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Flightless I (FliI) is the most evolutionarily conserved member of the gelsolin superfamily of proteins which are key regulators of actin filament assembly and turnover. FliI comprises an N-terminal leucine-rich repeat (LRR) domain which is not present in other gelsolin family members, and the LRR domain may enable interactions between FliI and other molecules involved in signal transduction, thereby spatially integrating signaling and actin remodeling functions. This protein was originally found in Drosophila and participates in the embryonic development, while mammalian FliI protein was involved in the regulation of wound repair,skin barrier development. Studies recently demonstrated that FliI protein associated with colorectal cancer, hepatocellular and prostate cancer (PMID:30091651; 28498392).