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Ferrostatin-1

CAS号

347174-05-4

分子式

C15H22N2O2

主要靶点

Antifungal|Ferroptosis

仅限科研使用

Cat No : CM00719

Print datasheet

Synonyms

Fungal|Fer1|Fer-1|Fer 1|Ferrostatin 1|Ferrostatin1|Ferrostatin-1|Ferrostatin-1 (Fer-1)|Ferroptosis|inhibit|cytosolic|death|cell|antifungal|antioxidant|Inhibitor|neurotoxicity|ROS|铁抑素-1

验证数据展示

  • CAS No.: 347174-05-4

    Ferrostatin-1
    CAS#: 347174-05-4

产品信息

CAS号 347174-05-4
分子式 C15H22N2O2
主要靶点 Antifungal|Ferroptosis
主要通路 凋亡|微生物学
分子量 262.35
纯度 99.68%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Fungal|Fer1|Fer-1|Fer 1|Ferrostatin 1|Ferrostatin1|Ferrostatin-1|Ferrostatin-1 (Fer-1)|Ferroptosis|inhibit|cytosolic|death|cell|antifungal|antioxidant|Inhibitor|neurotoxicity|ROS|铁抑素-1

靶点活性

Ferroptosis:60 nM(EC50, HT-1080 cells)

体内活性

方法：为研究铁死亡是否与 LPS 诱导的急性肾损伤 (AKI) 有关，将 Ferrostatin-1 (5 mg/kg) 单剂量腹腔注射给 C57BL/6 小鼠，30 min 后腹腔注射 LPS (10?mg/kg) 诱导感染性 AKI。 结果：Ferrostatin-1 显著保护小鼠免受 LPS 诱导的 AKI 中的肾功能障碍和肾小管损伤。[4] 方法：为研究铁紊乱是否与急性肝病的关系及其分子机制，将 Ferrostatin-1 (2.5 μM/kg) 腹腔注射给 ICR 小鼠，每天一次，持续三天，随后连续3天腹腔注射 TAA (250 mg/kg/天)，建立小鼠急性肝损伤 (ALI) 模型。 结果：Ferrostatin-1 预处理显著降低了 TAA 诱导的血浆 ALT、AST 和 LDH 水平的变化，抑制了 TfR1、Fpn 和 Ft-L 蛋白的表达，并减少了铁的积累，但不影响肝脏中 xCT 或 GPX4 的表达。Ferrostatin-1 通过降低肝脏中的铁含量来预防肝脏铁死亡。[5]

体外活性

方法：人支气管上皮细胞 BEAS-2B 用 LPS (10?mg/L) 和 Ferrostatin-1 (2 μM) 共同处理 16 h，使用 CCK-8 方法检测细胞生长抑制情况。 结果：Ferrostatin-1 可减轻LPS诱导的细胞损伤。[1] 方法：人纤维肉瘤细胞 HT-1080 用 Ferrostatin-1 (0.5 μM)和 Erastin (10 μM) 处理 4 h，使用 Flow Cytometry 方法检测细胞产生的 ROS 水平。 结果：Ferrostatin-1 抑制了 Erastin 诱导的胞质和脂质 ROS 的积累。[2] 方法：小鼠海马神经元细胞 HT-22 用 Ferrostatin-1 (3-12 μM) 处理 16 h 再加 5 mM glutamate 处理 24 h，检测 LDH 的释放水平。 结果：Glutamate 处理后 LDH 的释放显著增加，Ferrostatin-1 处理可抑制 LDH 的释放。[3]

溶解度

10% DMSO+40% PEG300+5% Tween 80+45% Saline:20 mg/mL (76.23 mM);Ethanol:26.2 mg/mL (100 mM);DMSO:45 mg/mL (171.53 mM)

细胞实验

Cell viability was typically assessed in 384-well format by Alamar Blue fluorescence (ex/em 530/590) measured on a Victor3 plate reader. In some experiments, Trypan Blue dye exclusion counting was performed using an automated cell counter. Cell viability under test conditions is reported as a percentage relative to the negative control treatment [1].

参考文献

1.Liu P, et al. Ferrostatin-1 alleviates lipopolysaccharide-induced acute lung injury via inhibiting ferroptosis. Cell Mol Biol Lett. 2020 Feb 27;25:10.
2.Dixon SJ, et al. Ferroptosis: an iron-dependent form of nonapoptotic cell death. Cell. 2012 May 25;149(5):1060-72.
3.Chu J, et al. Ferrostatin-1 protects HT-22 cells from oxidative toxicity. Neural Regen Res. 2020 Mar;15(3):528-536.
4.Tang Y, et al. Isoliquiritigenin attenuates septic acute kidney injury by regulating ferritinophagy-mediated ferroptosis. Ren Fail. 2021 Dec;43(1):1551-1560.
5.Jiang H, et al. Ferrostatin-1 Ameliorates Liver Dysfunction via Reducing Iron in Thioacetamide-induced Acute Liver Injury in Mice. Front Pharmacol. 2022 Apr 12;13:869794.
6.Chen X, Gao C, Cheng Z, et al. Ruxolitinib exerts neuroprotection via repressing ferroptosis in a mouse model of traumatic brain injury[J]. Experimental Neurology. 2021: 113762.
7.Yin W, Luan X, Li Z, et al. Structural basis for inhibition of the SARS-CoV-2 RNA polymerase by suramin[J]. Nature Structural & Molecular Biology. 2021: 1-7.
8.Wang S, Li F, Qiao R, et al. Arginine-Rich Manganese Silicate Nanobubbles as a Ferroptosis-Inducing Agent for Tumor-Targeted Theranostics[J]. ACS nano. 2018 Dec 26;12(12):12380-12392.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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