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验证数据展示

  • WB analysis of Jurkat using 13098-1-AP (same clone as 13098-1-PBS)

    WB analysis of Jurkat using 13098-1-AP (same clone as 13098-1-PBS)

    WB result of FAS/CD95 antibody (13098-1-AP, 1:1000) with si-control and si-FAS transfected Jurkat cells. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

    WB analysis using 13098-1-AP (same clone as 13098-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13098-1-AP (FAS/CD95 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 13098-1-AP (same clone as 13098-1-PBS)

    IP experiment of HeLa using 13098-1-AP (same clone as 13098-1-PBS)

    IP result of anti-FAS/CD95 (IP:13098-1-AP, 3ug; Detection:13098-1-AP 1:500) with HeLa cells lysate 3000ug. This data was developed using the same antibody clone with 13098-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Fas/CD95 Polyclonal antibody, PBS Only

Fas/CD95 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 13098-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13098-1-PBS targets Fas/CD95 in WB, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3718

Product name: Recombinant human FAS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 156-335 aa of BC012479

Sequence: ECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Fas (TNF receptor superfamily, member 6)
别名
计算分子量 35-38 kDa
观测分子量 38-45 kDa
GenBank蛋白编号BC012479
基因名称 Fas/CD95
Gene ID (NCBI) 355
RRIDAB_2278042
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25445
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The molecular mass of native Fas is 38 kDa, the high molecular weight form (40-55 kDa) of Fas is due to glycosylation.

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