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验证数据展示

  • WB analysis using 16609-1-AP

    WB analysis using 16609-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16609-1-AP (FTSJ3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 16609-1-AP

    WB analysis of MCF-7 using 16609-1-AP

    WB result of FTSJ3 antibody (16609-1-AP; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FTSJ3 transfected MCF-7 cells.

  • Featured Product
  • KD/KO验证

FTSJ3 Polyclonal antibody

FTSJ3 Polyclonal Antibody for WB, ELISA
Cat No. 16609-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC:2.1.1.-, EPCS3, FTSJ 3, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, SB92

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, SH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy FTSJ3 Ready-To-Use IHC Kit. FTSJ3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16609-1-AP targets FTSJ3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag9835

Product name: Recombinant human FTSJ3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 491-847 aa of BC036710

Sequence: KRMRLTEVQDDKEEEEEENPLLVPLEEKAVLQEEQANLWFSKGSFAGIEDDADEALEISQAQLLFENRRKGRQQQQKQQLPQTPPSCLKTEIMSPLYQDEAPKGTEASSGTEAATGLEGEEKDGISDSDSSTSSEEEESWEPLRGKKRSRGPKSDDDGFEIVPIEDPAKHRILDPEGLALGAVIASSKKAKRDLIDNSFNRYTFNEDEGELPEWFVQEEKQHRIRQLPVGKKEVEHYRKRWREINARPIKKVAEAKARKKRRMLKRLEQTRKKAEAVVNTVDISEREKVAQLRSLYKKAGLGKEKRHVTYVVAKKGVGRKVRRPAGVRGHFKVVDSRMKKDQRAQQRKEQKKKHKRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 FtsJ homolog 3 (E. coli)
别名 EC:2.1.1.-, EPCS3, FTSJ 3, pre-rRNA 2'-O-ribose RNA methyltransferase FTSJ3, SB92
计算分子量 847 aa, 97 kDa
观测分子量120-130 kDa
GenBank蛋白编号BC036710
基因名称 FTSJ3
Gene ID (NCBI) 117246
RRIDAB_3669252
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IY81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.

实验方案

Product Specific Protocols
WB protocol for FTSJ3 antibody 16609-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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