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验证数据展示

  • IHC staining of mouse thymus using 27319-1-AP

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AAGAB antibody included
  • IHC staining of mouse thymus using 27319-1-AP

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AAGAB antibody included
  • WB analysis of K-562 using 27319-1-AP

    WB analysis of K-562 using 27319-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27319-1-AP (AAGAB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AAGAB Polyclonal antibody

AAGAB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27319-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AAGAB, FLJ11506

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, mouse thymus tissue
Positive IHC detected inmouse thymus tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AAGAB Ready-To-Use IHC Kit. AAGAB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27319-1-AP targets AAGAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AAGAB fusion protein Ag26340 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alpha- and gamma-adaptin-binding protein p34
别名 AAGAB, FLJ11506
观测分子量 38 kDa
GenBank蛋白编号BC058886
基因名称 AAGAB
Gene ID (NCBI) 79719
RRIDAB_2880841
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PD74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Recent study indicate that mutations in AAGAB can cause punctate palmoplantar keratoderma type I (PPKP1; OMIM#148600), also called keratosis punctata palmoplantaris Buschke-Fischer-Brauer. The predicted MW of this protein is 35 kDa. Catalog#27319-1-AP recognises 37-40 kDa band may due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for AAGAB antibody 27319-1-APDownload protocol
IHC protocol for AAGAB antibody 27319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols