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验证数据展示

  • IHC staining of mouse thymus using 27319-1-AP

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AAGAB antibody included
  • IHC staining of mouse thymus using 27319-1-AP

    IHC staining of mouse thymus using 27319-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 27319-1-AP (AAGAB antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AAGAB antibody included
  • WB analysis of K-562 using 27319-1-AP

    WB analysis of K-562 using 27319-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 27319-1-AP (AAGAB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

AAGAB Polyclonal antibody

AAGAB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27319-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AAGAB, FLJ11506

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, mouse thymus tissue
Positive IHC detected inmouse thymus tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AAGAB Ready-To-Use IHC Kit. AAGAB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27319-1-AP targets AAGAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26340

Product name: Recombinant human FLJ11506 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-315 aa of BC058886

Sequence: MAAGVPCALVTSCSSVFSGDQLVQHILGTEDLIVEVTSNDAVRFYPWTIDNKYYSADINLCVVPNKFLVTAEIAESVQAFVVYFDSTQKSGLDSVSSWLPLAKAWLPEVMILVCDRVSEDGINRQKAQEWCIKHGFELVELSPEELPEEDDDFPESTGVKRIVQALNANVWSNVVMKNDRNQGFSLLNSLTGTNHSIGSADPCHPEQPHLPAADSTESLSDHRGGASNTTDAQVDSIVDPMLDLDIQELASLTTGGGDVENFERLFSKLKEMKDKAATLPHEQRKVHAEKVAKAFWMAIGGDRDEIEGLSSDEEH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alpha- and gamma-adaptin-binding protein p34
别名 AAGAB, FLJ11506
观测分子量 38 kDa
GenBank蛋白编号BC058886
基因名称 AAGAB
Gene ID (NCBI) 79719
RRIDAB_2880841
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PD74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Recent study indicate that mutations in AAGAB can cause punctate palmoplantar keratoderma type I (PPKP1; OMIM#148600), also called keratosis punctata palmoplantaris Buschke-Fischer-Brauer. The predicted MW of this protein is 35 kDa. Catalog#27319-1-AP recognises 37-40 kDa band may due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for AAGAB antibody 27319-1-APDownload protocol
IHC protocol for AAGAB antibody 27319-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols