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验证数据展示

  • WB analysis using 29749-1-AP

    WB analysis using 29749-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29749-1-AP (FGF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 29749-1-AP

    IF Staining of mouse brain using 29749-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FGF10 antibody (29749-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FGF10 Polyclonal antibody

FGF10 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 29749-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IF-P, ELISA

别名

FGF 10, FGF10, fibroblast growth factor 10, Keratinocyte growth factor 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29749-1-AP targets FGF10 in WB, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 FGF10 fusion protein Ag31278 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast growth factor 10
别名 FGF 10, FGF10, fibroblast growth factor 10, Keratinocyte growth factor 2
观测分子量40 kDa
GenBank蛋白编号BC105021
基因名称 FGF10
Gene ID (NCBI) 2255
RRIDAB_2918343
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15520
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGF10 belongs to the FGF7 subfamily. FGF10 is a paracrine signaling growth factor of 215 amino acids with a typical signal sequence for secretion and plays an essential role during development and tissue homeostasis in adults (PMID: 30405705). FGF10 is mainly produced by neuron and microglia/macrophages (PMID: 28981091). The calculated molecular weight of FGF10 is 23 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which may be a modified variant of FGF10.

实验方案

Product Specific Protocols
WB protocol for FGF10 antibody 29749-1-APDownload protocol
IF protocol for FGF10 antibody 29749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols