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FGF10 Polyclonal antibody

FGF10 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 29749-1-AP

Print datasheet

Synonyms

FGF 10, FGF10, fibroblast growth factor 10, Keratinocyte growth factor 2

验证数据展示

  • WB analysis using 29749-1-AP

    WB analysis using 29749-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29749-1-AP (FGF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 29749-1-AP

    IF Staining of mouse brain using 29749-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FGF10 antibody (29749-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29749-1-AP targets FGF10 in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IF-P, WB, ELISA
Tested Reactivity Human, Mouse
Immunogen FGF10 fusion protein Ag31278 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name fibroblast growth factor 10
Synonyms FGF 10, FGF10, fibroblast growth factor 10, Keratinocyte growth factor 2
Observed Molecular Weight40 kDa
GenBank Accession NumberBC105021
Gene Symbol FGF10
Gene ID (NCBI) 2255
RRIDAB_2918343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15520
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FGF10 belongs to the FGF7 subfamily. FGF10 is a paracrine signaling growth factor of 215 amino acids with a typical signal sequence for secretion and plays an essential role during development and tissue homeostasis in adults (PMID: 30405705). FGF10 is mainly produced by neuron and microglia/macrophages (PMID: 28981091). The calculated molecular weight of FGF10 is 23 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which may be a modified variant of FGF10.

实验方案

Product Specific Protocols
WB protocol for FGF10 antibody 29749-1-APDownload protocol
IF protocol for FGF10 antibody 29749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols