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验证数据展示

  • WB analysis using 30333-1-AP (same clone as 30333-1-PBS)

    WB analysis using 30333-1-AP (same clone as 30333-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30333-1-AP (FGF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30333-1-PBS in a different storage buffer formulation.

  • IF Staining of SKOV-3 using 30333-1-AP (same clone as 30333-1-PBS)

    IF Staining of SKOV-3 using 30333-1-AP (same clone as 30333-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using FGF-2 antibody (30333-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30333-1-PBS in a different storage buffer formulation.

FGF2 Polyclonal antibody, PBS Only

FGF2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30333-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

FGF-2, fibroblast growth factor 2, FGFB, FGF basic, FGF B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FGF2 Ready-To-Use IHC Kit. FGF2 primary antibody included.

产品信息

30333-1-PBS targets FGF2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28653

Product name: Recombinant human FGF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 143-275 aa of NM_002006

Sequence: MPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fibroblast growth factor 2 (basic)
别名 FGF-2, fibroblast growth factor 2, FGFB, FGF basic, FGF B
计算分子量 31 aa
观测分子量21 kDa，23 kDa
GenBank蛋白编号NM_002006
基因名称 FGF2
Gene ID (NCBI) 2247
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09038
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

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