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验证数据展示

  • WB analysis using 24754-1-AP (same clone as 24754-1-PBS)

    WB analysis using 24754-1-AP (same clone as 24754-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24754-1-AP (FBXL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24754-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 24754-1-AP (same clone as 24754-1-PBS)

    IF Staining of U2OS using 24754-1-AP (same clone as 24754-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using FBXL4 antibody (24754-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24754-1-PBS in a different storage buffer formulation.

FBXL4 Polyclonal antibody, PBS Only

FBXL4 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 24754-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

F box protein FBL4/FBL5, F box/LRR repeat protein 4, FBL4, FBL5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

24754-1-PBS targets FBXL4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21579

Product name: Recombinant human FBXL4 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 283-621 aa of BC055010

Sequence: LPYELIQLILNHLTLPDLCRLAQTCKLLSQHCCDPLQYIHLNLQPYWAKLDDTSLEFLQSRCTLVQWLNLSWTGNRGFISVAGFSRFLKVCGSELVRLELSCSHFLNETCLEVISEMCPNLQALNLSSCDKLPPQAFNHIAKLCSLKRLVLYRTKVEQTALLSILNFCSELQHLSLGSCVMIEDYDVIASMIGAKCKKLRTLDLWRCKNITENGIAELASGCPLLEELDLGWCPTLQSSTGCFTRLAHQLPNLQKLFLTANRSVCDTDIDELACNCTRLQQLDILGTRMVSPASLRKLLESCKDLSLLDVSFCSQIDNRAVLELNASFPKVFIKKSFTQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box and leucine-rich repeat protein 4
别名 F box protein FBL4/FBL5, F box/LRR repeat protein 4, FBL4, FBL5
计算分子量 621 aa, 70 kDa
观测分子量70 kDa
GenBank蛋白编号BC055010
基因名称 FBXL4
Gene ID (NCBI) 26235
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKA2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FBXL4 (F-box and leucine-rich repeat protein 4) is a mitochondrial protein that has been recently found to play significant roles in mtDNA maintenance (PMID: 28940506). Moreover, FBXL4 is a substrate receptor of a Cullin-1-RING E3 (CRL1) ligase complex that has previously been shown to bind to SKP1, the common CRL1 adapter subunit (PMID: 37102372).

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