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验证数据展示

  • IHC staining of mouse heart using 21437-1-AP

    IHC staining of mouse heart using 21437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21437-1-AP (FBXL21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C2C12 using 21437-1-AP

    IF Staining of C2C12 using 21437-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using FBXL21 antibody (21437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of mouse heart using 21437-1-AP

    IHC staining of mouse heart using 21437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21437-1-AP (FBXL21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 21437-1-AP

    IHC staining of mouse lung using 21437-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21437-1-AP (FBXL21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FBXL21 Polyclonal antibody

FBXL21 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 21437-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

F box/LRR repeat protein 21, F box/LRR repeat protein 3B, Fbl21, FBL3, FBL3B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse heart tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC2C12 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21437-1-AP targets FBXL21 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 FBXL21 fusion protein Ag16049 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 F-box and leucine-rich repeat protein 21
别名 F box/LRR repeat protein 21, F box/LRR repeat protein 3B, Fbl21, FBL3, FBL3B
计算分子量 434 aa, 49 kDa
GenBank蛋白编号BC106753
基因名称 FBXL21
Gene ID (NCBI) 26223
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3KPF8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FBXL21 (F-box and leucine-rich repeat protein 21) is a member of the F-box protein family, which plays a crucial role in regulating protein degradation through the ubiquitin-proteasome system. FBXL21 has been identified as a regulator in skeletal muscle. It mediates the rhythmic degradation of sarcomere proteins such as TCAP (titin cap) and MYOZ1 (Myozenin1) in conjunction with GSK-3β (PMID: 36574402).

实验方案

Product Specific Protocols
IHC protocol for FBXL21 antibody 21437-1-APDownload protocol
IF protocol for FBXL21 antibody 21437-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols