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验证数据展示

  • WB analysis using 26330-1-AP

    WB analysis using 26330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26330-1-AP (FAM134C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 26330-1-AP

    IF Staining of U2OS using 26330-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FAM134C antibody (26330-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FAM134C Polyclonal antibody

FAM134C Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26330-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

RETREG3, Reticulophagy regulator 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, HuH-7 cellls
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26330-1-AP targets FAM134C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23680

Product name: Recombinant human FAM134C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-127 aa of BC049370

Sequence: MAEAEGVPTTPGPASGSTFRGRRDVSGSWERDQQVEAAQRALVEVLGPYEPLLSRVQAALVWERPARSALWCLGLNAAFWFFALTSLRLVFLLAFGLMIIVCIDQWKNKIWPEIKVPRPDALDNESW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 family with sequence similarity 134, member C
别名 RETREG3, Reticulophagy regulator 3
观测分子量65 kDa
GenBank蛋白编号BC049370
基因名称 FAM134C
Gene ID (NCBI) 162427
RRIDAB_3085859
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VR2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The protein family with sequence similarity 134 (FAM134) comprises three proteins: FAM134B, FAM134A, and FAM134C. These proteins share the same LIR amino acid sequence. FAM134C, also known as the reticulophagy regulator 3 (RETREG3), plays a crucial role as an ER-phagy receptor. It is essential for maintaining ER morphology in a LC3-interacting region (LIR)-dependent manner.

实验方案

Product Specific Protocols
IF protocol for FAM134C antibody 26330-1-APDownload protocol
WB protocol for FAM134C antibody 26330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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