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验证数据展示

  • WB analysis using 12636-1-AP (same clone as 12636-1-PBS)

    WB analysis using 12636-1-AP (same clone as 12636-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12636-1-AP (same clone as 12636-1-PBS)

    IP experiment of HeLa using 12636-1-AP (same clone as 12636-1-PBS)

    IP result of anti-FAK (IP:12636-1-AP, 4ug; Detection:12636-1-AP 1:30000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 12636-1-AP (same clone as 12636-1-PBS)

    IF Staining of HUVEC using 12636-1-AP (same clone as 12636-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using FAK antibody (12636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

  • IHC staining of Hepatocellular carcinoma using 12636-1-AP (same clone as 12636-1-PBS)

    IHC staining of Hepatocellular carcinoma using 12636-1-AP (same clone as 12636-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FAK antibody included
  • Featured Product
  • KD/KO Validated

FAK Polyclonal antibody, PBS Only

FAK Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12636-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy FAK Ready-To-Use IHC Kit. FAK primary antibody included.

产品信息

12636-1-PBS targets FAK in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3331

Product name: Recombinant human FAK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 381-678 aa of BC028733

Sequence: ITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PTK2 protein tyrosine kinase 2
别名 PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1
计算分子量 1052 aa, 119 kDa
观测分子量 110 kDa
GenBank蛋白编号BC028733
基因名称 FAK
Gene ID (NCBI) 5747
RRIDAB_2173668
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05397
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

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