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验证数据展示

  • WB analysis using 34024-1-AP

    WB analysis using 34024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 34024-1-AP (FABP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse colon using 34024-1-AP

    IF Staining of mouse colon using 34024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse colon using 34024-1-AP

    IF Staining of mouse colon using 34024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat colon using 34024-1-AP

    IF Staining of rat colon using 34024-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using FABP1 antibody (34024-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FABP1 Polyclonal antibody

FABP1 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 34024-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat, pig

应用

WB, IF-P, ELISA

别名

14 kDa selenium-binding protein, Fabpl, Fatty acid-binding protein 1, Fatty acid-binding protein, liver, L-FABP

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue, rat colon tissue, rat liver tissue, pig liver tissue
Positive IF-P detected inmouse colon tissue, rat colon tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

34024-1-AP targets FABP1 in WB, IF-P, ELISA applications and shows reactivity with mouse, rat, pig samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 mouse, rat, pig
免疫原

CatNo: Eg7277

Product name: Recombinant rat FABP1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 2-127 aa of NM_012556.2

Sequence: NFSGKYQVQSQENFEPFMKAMGLPEDLIQKGKDIKGVSEIVHEGKKVKLTITYGSKVIHNEFTLGEECELETMTGEKVKAVVKMEGDNKMVTTFKGIKSVTEFNGDTITNTMTLGDIVYKRVSKRI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fatty acid binding protein 1, liver
别名 14 kDa selenium-binding protein, Fabpl, Fatty acid-binding protein 1, Fatty acid-binding protein, liver, L-FABP
计算分子量14 kDa
观测分子量14 kDa
GenBank蛋白编号NM_012556.2
基因名称 Fabp1
Gene ID (NCBI) 24360
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP02692
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 have been found to be elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).

实验方案

Product Specific Protocols
IF protocol for FABP1 antibody 34024-1-APDownload protocol
WB protocol for FABP1 antibody 34024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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