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验证数据展示

  • WB analysis using 32665-1-AP

    WB analysis using 32665-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32665-1-AP (Endoglin/CD105 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 32665-1-AP

    IHC staining of mouse liver using 32665-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32665-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 32665-1-AP

    IHC staining of mouse liver using 32665-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 32665-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Endoglin/CD105 Polyclonal antibody

Endoglin/CD105 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32665-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IHC, ELISA

别名

CD105, Edg, endoglin, Eng, S endoglin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inbEnd.3 cells, mouse kidney tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32665-1-AP targets Endoglin/CD105 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endoglin
别名 CD105, Edg, endoglin, Eng, S endoglin
计算分子量70kDa
观测分子量90 kDa
GenBank蛋白编号NM_007932.2
基因名称 CD105
Gene ID (NCBI) 13805
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ63961
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

实验方案

Product Specific Protocols
WB protocol for Endoglin/CD105 antibody 32665-1-APDownload protocol
IHC protocol for Endoglin/CD105 antibody 32665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols