验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MDA-MB-231 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86935-1-RR targets EVI1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag40447 Product name: Recombinant human EVI1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 813-1052 aa of BC130520 Sequence: HENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTDSLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSMARAAAESSAIQSISHV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ecotropic viral integration site 1 |
| 别名 | AML1 EVI 1, EC:2.1.1.367, EVI 1, EVI-1, MDS1 EVI1 |
| 计算分子量 | 1239 aa, 139 kDa |
| 观测分子量 | 118 kDa, 140 kDa |
| GenBank蛋白编号 | BC130520 |
| 基因名称 | EVI1 |
| Gene ID (NCBI) | 2122 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q03112 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Evi1 (ecotropic viral integration site 1) gene is an important proto-oncogene, which encodes a transcription factor with zinc finger structure, and this protein regulates the expression of other genes by binding to specific DNA sequences. EVI1 is highly expressed in hematopoietic stem cells (HSC) of human embryo and adult bone marrow, and rapidly down-regulated during differentiation. EVI1 knockout will lead to proliferation and regeneration defects of mouse HSC, accompanied by low expression of GATA2. Rebuilding the expression of EVI1 can restore the ability of proliferation and differentiation of mouse HSC by up-regulating GATA2. The two zinc finger domains of EVI1 can independently bind DNA and regulate different target genes, and they cooperate with FOS protein to jointly regulate cell adhesion, proliferation, colony formation and other processes. In advanced ovarian cancer, EVI1 and AP1 (activator protein 1, FOS is one of its main members) are both expressed abundantly.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EVI1 antibody 86935-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
