验证数据展示
经过测试的应用
| Positive WB detected in | bEnd.3 cells, mouse lung tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87684-1-RR targets ESAM in WB, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 |
CatNo: Eg6352 Product name: Recombinant mouse ESAM protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 30-251 aa of NM_027102.3 Sequence: QMELHVPPGLNKLEAVEGEEVVLPAWYTMAREESWSHPREVPILIWFLEQEGKEPNQVLSYINGVMTNKPGTALVHSISSRNVSLRLGALQEGDSGTYRCSVNVQNDEGKSIGHSIKSIELKVLVPPAPPSCSLQGVPYVGTNVTLNCKSPRSKPTAQYQWERLAPSSQVFFGPALDAVRGSLKLTNLSIAMSGVYVCKAQNRVGFAKCNVTLDVMTGSKAA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | endothelial cell-specific adhesion molecule |
| 别名 | Endothelial cell-selective adhesion molecule, Esam1 |
| 计算分子量 | 42 kDa |
| 观测分子量 | 42-60 kDa |
| GenBank蛋白编号 | NM_027102.3 |
| 基因名称 | Esam |
| Gene ID (NCBI) | 69524 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q925F2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ESAM, a 55 kDa type I transmembrane glycoprotein, possesses two extracellular Ig domains and a long cytoplasmic tail in which a PDZ domain is located (PMID: 22130718). The ~60 kDa band is glycosylated ESAM, and the lower band is deglycosylated ESAM.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ESAM antibody 87684-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
