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验证数据展示

  • WB analysis using 26468-1-AP

    WB analysis using 26468-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26468-1-AP (ERF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 26468-1-AP

    IP experiment of A431 using 26468-1-AP

    IP result of anti-ERF (IP:26468-1-AP, 4ug; Detection:26468-1-AP 1:2000) with A431 cells lysate 1040 ug.

ERF Polyclonal antibody

ERF Polyclonal Antibody for WB, IP, ELISA
Cat No. 26468-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Ets2 repressor factor, PE 2, PE2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, A549 cells, J774A.1 cells, RAW 264.7 cells
Positive IP detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26468-1-AP targets ERF in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24189

Product name: Recombinant human ERF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 138-323 aa of BC022231

Sequence: GGSHFRFPPSTPSEVLSPTEDPRSPPACSSSSSSLFSAVVARRLGRGSVSDCSDGTSELEEPLGEDPRARPPGPPDLGAFRGPPLARLPHDPGVFRVYPRPRGGPEPLSPFPVSPLAGPGSLLPPQLSPALPMTPTHLAYTPSPTLSPMYPSGGGGPSGSGGGSHFSFSPEDMKRYLQAHTQSVYN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ets2 repressor factor
别名 Ets2 repressor factor, PE 2, PE2
计算分子量 548 aa, 59 kDa
观测分子量 90 kDa
GenBank蛋白编号BC022231
基因名称 ERF
Gene ID (NCBI) 2077
RRIDAB_3669537
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50548
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ERF (Ets2 repressor factor) is also named as PE-2. Erf is a gene for a ubiquitously expressed Ets DNA-binding domain-containing transcriptional repressor regulated by Erk-dependent phosphorylation (PMID: 28694332, PMID: 35258130). Erf is involved in T cell maturation, acting as a positive regulator during CD4 and eventually CD8 lineage commitment, while negatively regulates the production of γδ T cells (PMID: 35258130). Erf is required for both primitive and erythromyeloid progenitor waves of hematopoietic stem cell (HSC)-independent hematopoiesis as well as for the normal function of HSCs (PMID: 28694332). ETS2 repressor factor (ERF) insufficiency causes craniosynostosis (CRS4) in humans and mice. ERF is an ETS domain transcriptional repressor regulated by Erk1/2 phosphorylation via nucleo-cytoplasmic shuttling (PMID: 37175668).

实验方案

Product Specific Protocols
IP protocol for ERF antibody 26468-1-APDownload protocol
WB protocol for ERF antibody 26468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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