EPZ015666

PRMT5:5 nM(Ki)

EPZ015666显示有效的细胞活性,阻断SmD3的对称二甲基化,并抑制MCL细胞系的增殖,IC50为96-904 nM。

口服200 mg/kg EPZ015666对MCL异种移植动物模型具有强的抗肿瘤活性.

Ethanol:45 mg/mL (117.36 mM);DMSO:45 mg/mL (117.36 mM)

EPZ015666 is dissolved in DMSO and stored, and then diluted with appropriate medium (final DMSO 0.2%) before use[1]. Cultured cells in linear/log-phase growth are split to a seeding density of 2×105 cells/mL in 2-20 mL of media, depending on the yield required at the end of the growth period. Compound is diluted in DMSO and added to each culture vessel with a final DMSO concentration of 0.2%. Cells are allowed to grow for 96 h undisturbed. At the conclusion of each treatment period, cells are harvested by centrifugation (5 min, 1,200 rpm), and cell pellets are rinsed once with PBS before being frozen on dry ice pending further processing. Long-term proliferation assays are performed on all MCL lines, with slight adjustments to initial seeding densities, depending on growth characteristics for each cell line. All assays are carried out for 12 d[1].