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验证数据展示

  • IHC staining of human placenta using 27443-1-AP

    IHC staining of human placenta using 27443-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 27443-1-AP

    IHC staining of human placenta using 27443-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27443-1-AP (EPB42 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 27443-1-AP

    WB analysis using 27443-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27443-1-AP (EPB42 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

EPB42 Polyclonal antibody

EPB42 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27443-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SPH5, Protein 4.2, PA, P4.2, Erythrocyte protein 4.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27443-1-AP targets EPB42 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag23556

Product name: Recombinant human EPB42 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 446-691 aa of BC096093

Sequence: EVLERVEKEKMEREKDNGIRPPSLETASPLYLLLKAPSSLPLRGDAQISVTLVNHSEQEKAVQLAIGVQAVHYNGVLAAKLWRKKLHLTLSANLEKIITIGLFFSNFERNPPENTFLRLTAMATHSESNLSCFAQEDIAICRPHLAIKMPEKAEQYQPLTASVSLQNSLDAPMEDCVISILGRGLIHRERSYRFRSVWPENTMCAKFQFTPTHVGLQRLTVEVDCNMFQNLTNYKSVTVVAPELSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 erythrocyte membrane protein band 4.2
别名 SPH5, Protein 4.2, PA, P4.2, Erythrocyte protein 4.2
观测分子量72 kDa
GenBank蛋白编号BC096093
基因名称 EPB42
Gene ID (NCBI) 2038
RRIDAB_3085962
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16452
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPB42, also known as Protein 4.2, is amongst the most abundant protein components of the erythrocyte membrane, representing approximately 5% membrane content or ~2.5 × 105 copies/cell. EPB42 binds to the cytoplasmic domain of band 3, which serves as a site for the anchorage of several membrane skeletal proteins(PMID: 19269200, 12049649). EPB42 is a 72 kDa peripheral membrane protein.

实验方案

Product Specific Protocols
WB protocol for EPB42 antibody 27443-1-APDownload protocol
IHC protocol for EPB42 antibody 27443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols