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验证数据展示

  • WB analysis of HepG2 using 18848-1-AP

    WB analysis of HepG2 using 18848-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18848-1-AP (ENTPD8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human normal stomach using 18848-1-AP

    IHC staining of human normal stomach using 18848-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 18848-1-AP (ENTPD8 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ENTPD8 Polyclonal antibody

ENTPD8 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18848-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

UNQ2492/PRO5779, NTPDase8, NTPDase 8, E-NTPDase 8, EC:3.6.1.5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman normal stomach
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18848-1-AP targets ENTPD8 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ectonucleoside triphosphate diphosphohydrolase 8
别名 UNQ2492/PRO5779, NTPDase8, NTPDase 8, E-NTPDase 8, EC:3.6.1.5
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号NM_198585
基因名称 ENTPD8
Gene ID (NCBI) 377841
RRIDAB_10597090
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5MY95
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENTPD8, also named as UNQ2492 and PRO5779, belongs to the GDA1/CD39 NTPase family. ENTPD8 is a canalicular ectonucleoside NTPDase which responsible for the main hepatic NTPDase activity. Ectonucleoside NTPDases catalyze the hydrolyzis of gamma- and beta-phosphate residues of nucleotides, playing a central role in concentration of extracellular nucleotides. Has activity toward ATP, ADP, UTP and UDP, but not toward AMP. ENTPD8 catalyze the reaction :ATP + 2 H2O = AMP + 2 phosphate.

实验方案

Product Specific Protocols
WB protocol for ENTPD8 antibody 18848-1-APDownload protocol
IHC protocol for ENTPD8 antibody 18848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols