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验证数据展示

  • WB analysis using 29712-1-AP (same clone as 29712-1-PBS)

    WB analysis using 29712-1-AP (same clone as 29712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29712-1-AP (EIF4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29712-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 29712-1-AP (same clone as 29712-1-PBS)

    IF Staining of HepG2 using 29712-1-AP (same clone as 29712-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4E antibody (29712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 29712-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29712-1-AP (same clone as 29712-1-PBS)

    IHC staining of human colon cancer using 29712-1-AP (same clone as 29712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29712-1-AP (EIF4E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29712-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4E antibody included

EIF4E Polyclonal antibody, PBS Only

EIF4E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29712-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.

产品信息

29712-1-PBS targets EIF4E in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E
别名
计算分子量 29 kDa
观测分子量 26-29 kDa
GenBank蛋白编号BC012611
基因名称 EIF4E
Gene ID (NCBI) 1977
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06730
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.

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