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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    WB analysis of HeLa using 10463-1-AP (same clone as 10463-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10463-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    IP experiment of HEK-293 using 10463-1-AP (same clone as 10463-1-PBS)

    IP result of anti-EIF4A3 (IP:10463-1-AP, 3ug; Detection:10463-1-AP 1:300) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 10463-1-PBS in a different storage buffer formulation.

EIF4A3 Polyclonal antibody, PBS Only

EIF4A3 Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 10463-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy EIF4A3 Ready-To-Use IHC Kit. EIF4A3 primary antibody included.

产品信息

10463-1-PBS targets EIF4A3 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0733

Product name: Recombinant human EIF4A3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of BC004386

Sequence: MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4A, isoform 3
别名
计算分子量 47 kDa
观测分子量 47 kDa
GenBank蛋白编号BC004386
基因名称 EIF4A3
Gene ID (NCBI) 9775
RRIDAB_2097394
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP38919
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions