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验证数据展示

  • WB analysis of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

    WB analysis of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11165-1-AP (EIF3G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

    IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3G antibody included
  • IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

    IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3G antibody included
  • IF Staining of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

    IF Staining of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 11165-1-AP (EIF3G antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

EIF3G Polyclonal antibody, PBS Only

EIF3G Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11165-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

eIF-3 RNA-binding subunit, eIF3 p44, eIF3 p42, eIF3 delta, eIF 3 RNA binding subunit

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF3G Ready-To-Use IHC Kit. EIF3G primary antibody included.

产品信息

11165-1-PBS targets EIF3G in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EIF3G fusion protein Ag1653 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit G
别名 eIF-3 RNA-binding subunit, eIF3 p44, eIF3 p42, eIF3 delta, eIF 3 RNA binding subunit
计算分子量 36 kDa
观测分子量 44 kDa
GenBank蛋白编号BC000733
基因名称 EIF3G
Gene ID (NCBI) 8666
RRIDAB_2096911
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75821
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3G is a component of the EIF3 complex. [PMID:20679478,20503360]