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验证数据展示

  • IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

    IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

    IHC staining of human ovary cancer using 27548-1-AP (same clone as 27548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27548-1-AP (ECSIT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

  • WB analysis using 27548-1-AP (same clone as 27548-1-PBS)

    WB analysis using 27548-1-AP (same clone as 27548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27548-1-AP (ECSIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27548-1-PBS in a different storage buffer formulation.

ECSIT Polyclonal antibody, PBS Only

ECSIT Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27548-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

SITPEC, Protein SITPEC, ECSIT homolog (Drosophila)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

27548-1-PBS targets ECSIT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag26877

Product name: Recombinant human ECSIT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 49-267 aa of BC008279

Sequence: SSEQSLVPSPPEPRQRPTKALVPFEDLFGQAPGGERDKASFLQTVQKFAEHSVRKRGHIDFIYLALRKMREYGVERDLAVYNQLLNIFPKEVFRPRNIIQRIFVHYPRQQECGIAVLEQMENHGVMPNKETEFLLIQIFGRKSYPMLKLVRLKLWFPRFMNVNPFPVPRDLPQDPVELAMFGLRHMEPDLSARVTIYQVPLPKDSTGAADPPQPHIVGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ECSIT homolog (Drosophila)
别名 SITPEC, Protein SITPEC, ECSIT homolog (Drosophila)
计算分子量 49 kDa
观测分子量49 kDa
GenBank蛋白编号BC008279
基因名称 ECSIT
Gene ID (NCBI) 51295
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQ95
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ECSIT is a cytosolic adaptor protein associated with the toll-like receptor pathway. It has a distinct N-terminal mitochondrial targeting sequence, pentatricopeptide repeat motif, and a C-terminal pleckstrin homology domain. ECSIT regulates many biological processes like embryonic development, inflammation, cardiac function, and assembly of mitochondrial complex I. In macrophages, the activation of TLRs by inflammatory signals promotes the translocation of TRAF6 to mitochondria where it engages ECSIT to stimulate the production of mitochondrial reactive oxygen species (ROS).

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