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验证数据展示

  • WB analysis using 27098-1-AP

    WB analysis using 27098-1-AP

    MDCK lysates were subjected to SDS PAGE followed by western blot with 27098-1-AP (ECHDC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 27098-1-AP

    IHC staining of human ovary cancer using 27098-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27098-1-AP (ECHDC3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ECHDC3 Polyclonal antibody

ECHDC3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27098-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, canine

应用

WB, IHC, ELISA

别名

PP8332, PP1494, Enoyl-CoA hydratase domain-containing protein 3, mitochondrial

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDCK cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27098-1-AP targets ECHDC3 in WB, IHC, ELISA applications and shows reactivity with human, canine samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, canine
免疫原

CatNo: Ag25707

Product name: Recombinant human ECHDC3 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 29-303 aa of BC009617

Sequence: RFCSRDPAGAGRRESEPRPTSARQLDGIRNIVLSNPKKRNTLSLAMLKSLQSDILHDADSNDLKVIIISAEGPVFSSGHDLKELTEEQGRDYHAEVFQTCSKVMMHIRNHPVPVIAMVNGLATAAGCQLVASCDIAVASDKSSFATPGVNVGLFCSTPGVALARAVPRKVALEMLFTGEPISAQEALLHGLLSKVVPEAELQEETMRIARKIASLSRPVVSLGKATFYKQLPQDLGTAYYLTSQAMVDNLALRDGQEGITAFLQKRKPVWSHEPV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 enoyl Coenzyme A hydratase domain containing 3
别名 PP8332, PP1494, Enoyl-CoA hydratase domain-containing protein 3, mitochondrial
观测分子量 33 kDa
GenBank蛋白编号BC009617
基因名称 ECHDC3
Gene ID (NCBI) 79746
RRIDAB_3085926
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96DC8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for ECHDC3 antibody 27098-1-APDownload protocol
WB protocol for ECHDC3 antibody 27098-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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